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KMID : 1001920090460040397
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Journal of Korean Neurosurgical Society
2009 Volume.46 No. 4 p.397 ~ p.402
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Evaluation of Spinal Fusion Using Bone Marrow Derived Mesenchymal Stem Cells with or without Fibroblast Growth Factor-4
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Seo Hyun-Sung
Jung Jong-Kwon
Lim Mi-Hyun
Hyun Dong-Keun
Oh Nam-Sik
Yoon Seung-Hwan
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Abstract
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Objective: In this study, the authors assessed the ability of rat bone marrow derived mesenchymal stem cells (BMDMSCs), in the presence of a growth factor, fibroblast growth factor-4 (FGF-4) and hydroxyapatite, to act as a scaffold for posterolateral spinal fusion in a rat model.
Methods: Using a rat posterolateral spine fusion model, the experimental study comprised 3 groups. Group 1 was composed of 6 animals that were implanted with 0.08 gram hydroxyapatite only. Group 2 was composed of 6 animals that were implanted with 0.08 gram hydroxyapatite containing 1 × 106/ 60 μL rat of BMDMSCs. Group 3 was composed of 6 animals that were implanted with 0.08 gram hydroxyapatite containing 1 × 106/ 60 μL of rat BMDMSCs and FGF-4 1 μG to induce the bony differentiation of the BMDMSCs. Rats were assessed using radiographs obtained at 4, 6, and 8 weeks postoperatively. After sacrifice, spines were explanted and assessed by manual palpation, high-resolution microcomputerized tomography, and histological analysis.
Results: Radiographic, high-resolution microcomputerized tomographic, and manual palpation revealed spinal fusion in five rats (83%) in Group 2 at 8 weeks. However, in Group 1, three (60%) rats developed fusion at L4-L5 by radiography and two (40%) by manual palpation in radiographic examination. In addition, in Group 3, bone fusion was observed in only 50% of rats by manual palpation and radiographic examination at this time.
Conclusion: The present study demonstrates that 0.08 gram of hydroxyapatite with 1 × 106/ 60 μL rat of BMDMSCs induced bone fusion. FGF-4, added to differentiate primitive 1 × 106/ 60 μL rat of BMDMSCs did not induce fusion. Based on histologic data, FGF-4 appears to induce fibrotic change rather than differentiation to bone by 1 × 106/ 60 μL rat of BMDMSCs.
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KEYWORD
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Bone marrow derived mesenchymal stem cell, Fibroblast growth factor-4, Hydroxyapatite, Spinal fusion
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